7/22/2023 0 Comments Noti hf nebFoot-and-mouth disease virus (FMDV), the causative agent of FMD, is a member of the Picornaviridae family, and has an icosahedral capsid composed of four structural proteins, VP1, VP2, VP3, and VP4, derived from the processing of the FMDV P1 polypeptide by viral 3C protease. Further work is needed to develop these DNA plasmid-based constructs into standalone FMD vaccines in cattle.įoot-and-mouth disease (FMD) is a highly contagious vesicular disease affecting both domestic livestock, including swine and cattle, and wildlife. Administration of pTarget O1P1-3C plasmid enhanced neutralizing antibody titers when used as a priming dose prior to administration of a conditionally licensed adenovirus-vectored FMD vaccine. ![]() Despite VLP formation in vitro, none of the DNA vaccine candidates elicited protection from clinical disease when administered independently. Three constructs, O1P1-3C minicircles, pTarget O1P1-3C, and mpTarget O1P1-3C LT plasmids, produced intracellular VLP crystalline arrays detected by electron microscopy. All constructs produced mature FMDV P1 cleavage products in transfected cells, as seen by western blot analysis. A modified pTarget plasmid with a reduced backbone size, mpTarget O1P1-3C LT, used a 3C protease containing two mutations reported to enhance expression. Both the pTarget O1P1-3C plasmid and O1P1-3C minicircle encoded a wild-type FMDV 3C protease to process the P1-2A polypeptide, whereas the O1P1-HIV-3C T minicircle used an HIV-1 ribosomal frameshift to down-regulate expression of a mutant 3C protease. ![]() Two traditional DNA plasmids and two DNA minicircle constructs were evaluated. We evaluated four DNA vaccine candidates for their ability to produce virus-like particles (VLPs) and elicit a protective immune response against Foot-and-mouth disease virus (FMDV) in cattle.
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